Establishment and neurochemical studies of animal models with anterior transposition of the ulnar nerve
10.3760/cma.j.issn.1671-8925.2010.03.010
- VernacularTitle:尺神经前置动物模型的建立及其神经生化研究
- Author:
Ya-Zhong ZHU
1
;
Sui-Pei WU
;
Li-Xin YU
;
Rong ZHOU
;
Xin-Hong ZHU
;
De-Sheng DONG
;
Shu-Cai LING
;
Chun-Sheng WANG
Author Information
1. 解放军第98医院
- Keywords:
Ulnar nerve anterior transposition;
Animal model;
Neurochemistry
- From:
Chinese Journal of Neuromedicine
2010;9(3):258-261
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish animal models with anterior transposition of the ulnar nerve and evaluate the safety of anterior transposition of the ulnar nerve at molecular level.Methods Location of the ulnar nerve of elbow in 5 rats were found similar to human being by anatomy.Twenty healthy adult SD rats,weighting about 250 g,were performed the anterior transposition of the ulnar nerve in the right forelimbs and the left forelimbs was considered as control group.The bilateral flexor carpi ulnaris muscles were weighed and the slice of cervical spinal cord(C_6-T_1)level were prepared 1 month after the operation.Nissl staining,NADPH-d histochemical staining,IB4 staining and ChAT-immunohistochemical staining were employed to observe the spinal cord(C_6-T_1)level at molecular level;electron microscope was used to observe the ultrastructure of ChAT-positive neurons.Statistical analysis was paired T test.Results The flexor carpi ulnaris muscles in the model group(92.3±9.13mg)and control group(93.2±7.29 mg)were not significantly different(P>0.05).After anterior transposition of the ulnar nerve in rats,no significant differences in cell number and morphology in the cervical spinal cord(C_6-T_1)were found between the model group and the control group(P>0.05).No changes between the 2 groups were noted in the fine structure of anterior horn motor neurons and the expression of nenrotransmitters(P>0.05).Conclusion Anterior transposition of the ulnar nerve can be safely done in the animal models(rats).
