Cyclosporin A affects the level of anti-myelin basic protein in serum and myelinoclasis after rat brain trauma
10.3760/cma.j.issn.1671-8925.2010.03.003
- VernacularTitle:环孢素A对大鼠脑损伤后髓鞘碱性蛋白抗体及继发性脱髓鞘病变的影响
- Author:
Jian-Cheng CHU
1
;
Wei LI
;
Ling-Hui LIU
;
Hong LI
;
Shan-Cheng CHENG
Author Information
1. 暨南大学附属第一医院
- Keywords:
Cyclosporin A;
Myelin basic protein;
Myelinoclasis
- From:
Chinese Journal of Neuromedicine
2010;9(3):227-230
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of cyclosporin A(CsA)on the level of anti-myelin basic protein(anti-MBP)in the serum and myelinoclasis in rat models after brain trauma.Methods Seventy-seven SD rats were randomized into blank control group(n=7),model group(n=35)and CsA-treated group(n=35).The 0.9% normal saline(5 mg/kg/d)was intraperitoneally injected into the model group and 5 mg/mL CsA(5 mg/kg/d)was injected into the CsA-treated group.Different time points(1,4,10,20 and 30 d)after the brain trauma,the MBP content and anti-MBP titer in the serum were measured using enzyme-linked immunosorbent assay(ELISA)and the degree of myelinoclasis in the brain stem slices was assessed with Marchi's method.Results As compared with that in the blank control group,the MBP and Anti-MBP contents in the model group were significantly increased(P<0.05):anti-MBP in the serum and the degree of myelinoclasis in the brain stem were all significantly increased in the 4th d,both reaching their highest levels on 10~(th) d.No significant differences of the MBP content in the serum between the CsA-treated group and the model group were found the same time points after the injury(P>0.05).However,lower level of anti-MBP in the serum 1 d after the injury and degree of myelinoclasis in the brain stem 4 daRer the injury in the CsA-treated group were observed as compared with those in the model group(P<0.05).Statistical analysis showed an intimate positive correlation between anti-MBP titer in serum and the degree of rnyelinoclasis(r=0.959,P=0.000).Conclusion CsA can affect the immune system to decrease the degree of myelinoclasis in the brain stem through decreasing the level of anti-MBP in the serum of rat models after brain trauma.
