Protective mechanism of insulin on paraquat-induced PC12 cells
10.3760/cma.j.issn.1671-8925.2010.02.007
- VernacularTitle:胰岛素对百草枯诱导损伤PC12细胞的保护机制的研究
- Author:
Xin-Ling YANG
1
;
Jian CAI
;
Ya-Ni YAO
;
Jian-Jun WU
;
Yu-Ping JIANG
;
Chen ZHANG
Author Information
1. 新疆医科大学附属第一医院
- Keywords:
Parkinson's disease;
Paraquat;
Insulin;
Phosphorylation protein INR Tyr~(1162/1163);
Phosphorylation protein AktSer~(473)
- From:
Chinese Journal of Neuromedicine
2010;9(2):137-140
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the protective mechanism of insulin on paraquat-induced PC12 cells. Methods Based on the Part (insulin can protect the paraquat-induced PC12 cells), immunoprecipitation and Western blotting were employed to observe the protein expressions of INR Tyr~(1162/1163) and AktSer~(473) in the group of normal PC12 cells, group of insulin interfered PC12 cells, group of PQ-induced PC12 cells and group of PQ combined with insulin interfered PC12 cells, respectively. Results Group of insulin interfered PCI2 cells and group of PQ combined with insulin interfered PC12 cells showed expression of phosphorylation protein INR Tyr~(1162/1163), while the other groups without insulin intervention did not show the expression of that. The expression of phosphorylation protein INR Tyr~(1162/1163) in the group of insulin interfered PC12 cells was higher than that in the group of PQ combined with insulin interfered PC12 cells, but statistical analysis showed no significant difference between the 2 groups (P>0.05). Meanwhile, the expression of phosphorylation protein AktSer~(473) in the group of insulin interfered PC 12 cells was obviously higher than that in the group of PQ combined with insulin interfered PC12 cells (P<0.05). Conclusion The increased expression of phosphorylation protein INR Tyr~(1162/1163) and AktSer~(473) might be one of the clues in proving that insulin may have the protective effect on PC12 cells.
