Effects of transient axonal glycoprotein-1 on activity of U251 cells and it's genetic regulatory mechanism
10.3760/cma.j.issn.1671-8925.2010.02.001
- VernacularTitle:TAG-1对U251细胞活力的影响及相关基因调节机制研究
- Author:
Hai-Gang CHANG
1
;
Xiao-Dan JIANG
;
Zhong-Can CHEN
;
Lu-Jun YANG
;
Zhi-Qiang FA
;
Mou-Xuan DU
Author Information
1. 广东省脑功能修复与再生重点实验室,广东省神经外科研究所,南方医科大学珠江医院神经外科,广州,510282
- Keywords:
Transient axonal glycoprotein-1;
APP intracellualr domain;
Glioma;
Apoptosis
- From:
Chinese Journal of Neuromedicine
2010;9(2):109-114
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of transient axonal glycoprotein-1 (TAG-1) on activity of U251 cells and expressions of AICD, p53 and EGFR genes in the cells. Methods The viability of U251 cells was tested by MTT assay at 48 h following the addition of various concentrations of TAG-1 (0, 5, 10 and 20 μg/mL). The expression ofamyloid precursor protein (ALP) was detected by immunofluorescent staining. The apoptotic cells were examined by TUNEL. Real-time PCR was employed to detect the influence of TAG-1 on the expressions of AICD, p53 and EGFR genes in U251 cells. Results TAG-1 did not play an inhibitory effect on the proliferation of the U251 cells. APP was abundantly expressed on membrane of the U251 cells. U251 cells did not show apoptotic cells but increased expressions of AICD, p53 and EGFR genes were noted when U251 cells were exposed at 10 μg/mL of TAG-1. Conclusion TAG-1 plays an important role in regulating the proliferation of glioma and may not induce the apoptosis of U251 cells through the signal pathway of TAG-1/APP/AICD/p53 or TAG- 1/APP/AICD/EGFR.
