Effects of intracerebroventricular administration of brain-derived neurotrophic factor at different time intervals on cerebral ischemia-reperfusion injuries in rats
10.3760/cma.j.issn.1671-8925.2010.01.009
- VernacularTitle:不同时间脑室注射BDNF对大鼠脑缺血再灌注损伤的保护作用
- Author:
Yong-Xing TAN
1
;
Xue-Mei LI
;
Jun-Xiong YU
;
Gao-Xiang LIN
;
Yi-Hong JIANG
Author Information
1. 桂林医学院附属医院
- Keywords:
Brain-derived neurotrophic factor;
Brain ischemia-reperfusion;
Superoxide dismutase;
Malondialdehyde;
Apoptosis
- From:
Chinese Journal of Neuromedicine
2010;9(1):38-42
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the effects of intracerebroventricular administration of exogenous brain-derived neurotrophie factor (BDNF) at different intervals on cerebral ischemia-reperfusion(I/R)injuries in rats. Methods A total of 70 adult male Wistar rats,weighring 250-300g,were randomly divided into normal control group(group C,n=10),ischemia-reperfusion group(group I/P,n=10)and BDNF-treatment groups([12 or 6 h before ischemia,at just the moment performing ischemia,60r12 h after ischemia][group A1,A2,A3,A4 and A5],n=10).Focal cerebral I/R model was established in rats by reversible left middle cerebral artery occlusion with filament.BDNF(0.5μg)was microinjected into the left lateral ventricular of the rats in the BDNF-treatment groups.The activities of superoxide dismutase(SOD)in the brain tissue were measured by xanthine oxidase method and the contents of malondialdehyde(MDA)in the brain tissue were determined by thiobarbituric acid method.Neuron apoptosis in the brain cortex was identified by TUNEL.One left brain cortex(1 mmx×1mm)was removed randomly in each group to observe the ultrastructure changes of cerebral tissue under electron microscope. Results Compared with those in the group I/R,the activity of SOD was increased significantly but the content of MDA was decreased significantly in ischemia brain tissue in every BDNF-treatmnent group (P<0.05).The index of apoptotic neuron cells in the BDNF-treatment groups was decreased significantly as compared with that in the group I/R(P<0.05).The activity of SOD in the brain tissue(25.02±2.77,24.01±1.03)was higher and the content of MDA(10.35±1.23,12.29±0.92)and the index of neuron apoptosis(21.77±3.56.23.84±2.63)were lower in the BDNF-treatment groups than those in the other 2 groups at 12 or 6 h before ischemia (P<0.05).The ultrastructure of the cerebral tissue in each BDNF treatment group was improved to different degree,but that in I/R group was seriously damaged. Conclusion Intracerebroventricular administration of BDNF at different time intervals, significantly decreasing the oxidative stress and the neuron apoptosis rate,has obvious protective effect in a time-dependent manner on brain injury and intracerebroventricular microinjection of BDNF before ischemia has a far better effect.
