Relationship between cognitive ability and both thyroid function and mRNA level of thyroid hormone receptor α1 in brain tissue of rats with nonthyroidal illness syndrome
10.3760/cma.j.issn.1671-8925.2010.01.008
- VernacularTitle:非甲状腺疾病大鼠中枢甲状腺功能及受体α1 mRNA水平与认知能力的研究
- Author:
Da-Hua YUAN
1
;
Qun WANG
Author Information
1. 南方医科大学南方医院
- Keywords:
Nonthyroidal illness syndrome;
Thyroid hormone;
Cognition;
Thyroid hormone receptor
- From:
Chinese Journal of Neuromedicine
2010;9(1):34-37,42
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the changes of cognitive ability and thyroid function and mRNA level of thyroid hormone receptor α1(TRα1)in the brain tissues of rats with nonthyroidal illness syndrome (NTIS). Methods Nineteen adull male SD rats were randomly divided into normal control (n=9)and NTIS(n=10)groups.The NTIS model,its liver been fiberized was induced by intraperitoneal injecting 0.15 mL carbon tetrachloride([CCL4],[1:6,diluted by com oil])3 times per week with duration of 8 weeks.The normal control group was injected equal sodium.Morris water-maze task was employed to test the cognitive memory ability of the rats;the concentrations of T_3,T_4, FT_3,FT_4,rT_3 and TSH in the blood serum and T_3,T_4 and rT_3 in the brain tissue were detected by using radioimmunoassay(RIA);the mRNA level of TRα1 was detected by fluorescent quantitative RT-PCR. Resuits Morris water-maze task showed no significant difference in congnitive ability between NTIS and control groups (P>0.05).The concentrations of T_4,FT_3,FT_4 and T_3 in blood serum in the NTIS group were significantly lower as compared with those in the normal control group(P<0.05).Concentrations of rT3,TSH in the blood serum,the concentrations of T_4,T_3,rT_3 and the mRNA level of TRα1 in the brain tissue had no significant differences between the two groups(P>0.05). Conclusion The rats with hepatic fibrosis showed typical NTIS.Thyroid function in central nervous system of these rats is normal, so is their cognitive ability.The mRNA level of TRα1 had no significant change in brain tissue of these rats.
