Dynamic changes of estradiol and pregnenolone levels in serum and hippocampus of female rats with epilepsy
10.3760/cma.j.issn.1671-8925.2010.01.007
- VernacularTitle:致痫后雌性大鼠血清及海马雌二醇和孕烯醇酮水平的动态变化
- Author:
Yu ZONG
1
;
Yu MA
;
Hao-Jie LIN
;
Xin WANG
Author Information
1. 复旦大学附属中山医院
- Keywords:
Estradiol;
Pregnenoloene;
Serum;
Hippocampus;
Epileptic seizure
- From:
Chinese Journal of Neuromedicine
2010;9(1):29-33
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the dynamic changes of estradiol(E2)and pregnenolone (PREG)levels in serum and hippocampus of female rats with epilepsy and explore the relationship between E2 level in the hippocampus and severity of seizure. Methods The seizure model was established by injecting kainite(KA)into the hippocampus of amygdala-kindled rats.The behaviors at the moment of epileptic seizure were observed and serum and hippocampus samples were collected at 0,1,2,3,4,5,6,12 and 24 h after seizure.Rats in the control group was injected the same volume of saline at the sanle time points.The levels of E2 and PREG in the Seizure and control groups were measured by radioimmunoassay and enzyme linked immunosorbent assay,respectively.These results were statistically analyzed. Results Epileptic Seizure was showed after KA injection 5 to 10 min with the strongest seizure appearing at 3 h.The level of E2 was not significantly different in serum of the rats with epileptic seizure.While the level of E2 significantly changed in the hippocampal tissue after seizure:it elevated 1h after epileptic seizure,peaked at 4 h and returned to normal at 12 h;statistically significant differences of E2 level in the hippocampai tissue was showed among different time points(P<0.05).Besides,the E2 level in the hippocampus was increased gradually with the aggravation of the seizure(R~2=0.646,P<0.05).No significant differences were found in the PREG level of the hippocampal tissues between the rats treated with KA and the rats in the control group. Conclusion Epileptic seizure can induce local E2 synthesis in the rat hippocampus,thus,the E2 level in the hippocampus and severity of seizure is positively correlative.
