Alteration of ATP-sensitive K~+ channel expression in hippocampal neurons after severe chronic hypoxia
10.3760/cma.j.issn.1671-8925.2010.01.002
- VernacularTitle:重度缺氧中海马神经元KATP通道的表达改变
- Author:
Lian-Yan HUANG
1
;
Bo-Xing LI
;
Xiao-Zhou HUANG
;
A-Dan SHEN
;
Fei ZOU
Author Information
1. 南方医科大学
- Keywords:
ATP-sensitive K~+ channel;
Severe hypoxia;
Hippocampal neurons
- From:
Chinese Journal of Neuromedicine
2010;9(1):7-10
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the alteration of ATP-sensitive K~+(K_(ATP))channel expression in hippocampal neurons after severe chronic hypoxia. Methods The hippocampal neurons from 1-week-old rat were incubated and divided into normal control(incubated under 5%CO_2 and 95%air for 8h),hypoxia(incubated under 5%CO2 and 95%N_2 for 8h),hypoxia combined with diazoxide-treated (incubated with 100 μmol/L diazoxide under 5%CO_2 and 95%N_2 for 8h)and hypoxia combined with tolbutamide-treated groups(incubated with 100 μmol/L tolbutamide under 5%CO_2 and 95%N_2 for 8h).Cell apopmsis was identified by MTT.And the mRNA and protein expressions of K_(ATP) channel were estimated by RT-PCR and Western blot analysis.respectively. Results Hypoxia combined with diazoxide-treated group showed a significantly decreased apoptosis rate of neuron as compare with the normal control group 8h after hypoxia(P<0.05);while hypoxia combined with tolbutamide-treated group showed a significantly increased apoptosis rate of neuron compared with the normal control group(P<0.05).The expression of SUR1 in the three hypoxia groups significantly increased as compared with that in the normal control group(P<0.05);however,the expression of Kir6.2 in the three hypoxia groups did not change as compared with that in the normal control group(P>0.05).Conclusion K(ATP),channels can protect the hippocampal neurons under severe chronic hypoxia through the activation of KATP channels and upregulation of expression of KATP channels SUP1 subunit.
