A novel VEGF16S-PE38 fusion gene for anti-angiogenic therapy in malignant glioma of nude mice model
10.3760/cma.j.issn.1671-8925.2009.12.004
- VernacularTitle:重组VEGF165-PE38融合基因对裸鼠移植性人胶质瘤血管生成的抑制作用
- Author:
Yi-Quan KE
1
;
Chang-Chen HU
;
Xiao-Dan JIANG
;
Yu-Sheng WANG
;
Xin-Lin SUN
;
Sha XUE
;
Fa-Bing ZHANG
;
Peng-Fei YAO
;
Ying-Qian CAI
Author Information
1. 南方医科大学珠江医院
- Keywords:
Vascular endothelial cell growth factor receptor;
Pseudomonas exotoxin A;
Fusion gene;
Gene therapy;
Anti-angiogenic therapy;
Glioma
- From:
Chinese Journal of Neuromedicine
2009;8(12):1203-1206
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate the anti-angiogenic effect of eukaryotic vector containing human VEGF 165 and truncated pseudomonas exotoxin A (PE38) fusion gene on malignant glioma in nude mice, and explore a novel anti-angiogenic therapy for cancer. Methods The models were established through hypodermic injection of human U251 glioma cells into the nude mice and randomly divided into untreated group, PBS group, pIRES2-EGFP group and pIRES2-VEGF165-PE38-EGFP group on the 9th day. H&E staining was performed to observe the morphological changes of the glioma tissues; SP immunohistochemistry was employed to detect the expression of GFAP, CD31 andPE; quantitative pathologic image analysis system was used to investigate the microvessel density (MVD) in the tumor tissues. Results At day 16, the pIRES2-VEGF165PE38-EGFP group showed significantly lower tumor volume of mice and significantly decreased MVD than the other three groups (P<0.05). Positive expression of PE was shown in the pIRES2-VEGF165PE38-EGFP group, but negative in the other three groups. Conclusion The expression products of VEGFJ65-PE38 fusion gene can obviously inhibit the growth and angiogenesis of U2S1 cells in nude mouse flank tumor models, suggesting that it may be a novel therapeutic approach for anti-angiogenic therapy of cancer.
