Effect of Tenuigenin on Mash1 expression and directional differentiation of neural stem cells from mouse hippocampus in vitro
10.3760/cma.j.issn.1671-8925.2009.12.002
- VernacularTitle:远志总皂苷对海马神经干细胞定向分化的作用及对Mash1表达的影响
- Author:
Xiao-Feng ZHU
1
;
Xun-Zhong QI
;
Jing SHI
;
Jin-Cheng ZHA
;
Jin-Zhou TIAN
Author Information
1. 北京中医药大学东直门医院
- Keywords:
Neural stem cell;
Cholinergic neuron;
Tenuigenin;
Mashl
- From:
Chinese Journal of Neuromedicine
2009;8(12):1193-1196,1202
- CountryChina
- Language:Chinese
-
Abstract:
Objective To determine the effect of Tenuigenin (TEN) on the Mashl expression in neural stem cells (NSCs) from mouse hippocampus and directional differentiation of its NSCs in vitro. Methods NSCs were generated from the mouse hippocampus within 24 hours after birth and cultured with EGF and bFGF (20 ng/mL, each) in a 50-mm uncoated culture flask. The third-passage NSCs were cultured with TEN medium and divided them into vehicle, 5 mg/L, 20 mg/L and 100 mg/L groups according to different TEN concentrations. Immunocytochemical techniques were employed to detect the NSE-positive neurons and ChAT-positive neurons induced by TEN, and the expression of Mash1 in NSCs affected by TEN was measured by RT-PCR techniques. Results The immunocytochemical and RT-PCR results revealed that ChAT-positive neurons and NSE-positive neurons were detected with statistical significance and 20 mg/L group showed a stronger effect than other concentration groups (P< 0.05). The expression of Mash1 in the 20 mg/L group increased as compared with that in the vehicle group (P<0.05). Conclusion TEN can induce NSCs in vitro differentiating into neurons and cholinergic neurons, and increase the expression of Mashl in NSCs.
