Effect of 4-hydroxytamoxifen on the expression of pituitary tumor transforming gene in GH3 prolactinonm cells
10.3760/cma.j.issn.1671-8925.2009.11.006
- VernacularTitle:4-羟基他莫昔芬对泌乳素腺瘤GH3细胞PTTG表达的影响
- Author:
You-Qiang CUI
1
;
Jian-Jun WANG
;
Liang-Zhu TENG
;
Jian-Xin KONG
;
Meng LI
;
Jian GUO
;
Jin-Long SUN
Author Information
1. 山东省千佛山医院
- Keywords:
Prolactinomas;
Pituitary tumor transforming gene;
4-hydroxytamoxifen
- From:
Chinese Journal of Neuromedicine
2009;8(11):1103-1105,1110
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of 4-hydroxytamoxifen on the expression of pituitary tumor transforming gene (PTTG) in GH3 prolactinoma cells. Methods RT-PCR and Western blotting were employed to detect the expressions of PTTG mRNA and protein in human GH3 prolaetinoma cells. Different concentrations of estradiol (E2) or 4-hydroxytamoxifen (OHTam) were addedl into the hormone-depleted medium, and the viable cell number and expression levels of PTTG mRNA and protein were measured. Results The growth of OH3 prolaetinoma cells was significantly inhibited in hormone-depleted medium. E2 at a concentration of 1×10<'-8> mol/L obviously promoted the cell growth, the effect of which was inhibited by the application of OHTam (1×10<'-6> mol/L) to cause slowed cell growth. The expressions of PTTG at both the mRNA and protein levels were detected in detected in untreated GH3 prolactinoma cells, and OHTam at the concentration of 1×10<'-6> mol/L significantly inhibited their expressions. Conclusion The growth of GH3 cells is estrogen-dependant and can be inhibited by estrogen antagonist OHTam, which also results in reduced expression of PTTG gene in the cells.
