Effect of brain homogenate from hypoxia-preconditioned mice on rat embryonic hippocampal neurons with hypoxia/reoxygenation injury
10.3760/cma.j.issn.1671-8925.2009.11.004
- VernacularTitle:低氧预适应小鼠脑匀浆液提取液对大鼠鼠胚海马神经元缺氧复氧后神经细胞的影响
- Author:
Ming-Feng YANG
1
;
Yan-Bo ZHANG
;
Bao-Liang SUN
;
Jing-Zhong NIU
;
Guo-Wei LU
Author Information
1. 泰山医学院附属医院
- Keywords:
Hypoxia preconditioning;
Hypoxia-reoxygenation;
Apoptosis;
Hippocampus
- From:
Chinese Journal of Neuromedicine
2009;8(11):1094-1097
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the effect of brain homogenate (BH) extracted from hypoxia-preconditioned mice on the viability and apoptosis of rat embryonic hippocampal neurons with hypoxia/reoxygenation-induced injury. Methods Rat embryonic hippocampal neurons primarily cultured for 8 days in 96 well tissue culture plate were divided into 5 groups, namely the normal control group (treated with PBS), H<,4>R<,48> group (with hypoxia for 4 h followed by reoxygenation for 48 h and PBS treatment), H0 group (with BH from normal mice prior to hypoxia/reoxygenation), H1 group (with BH from acute hypoxia-preconditioned mice and hypoxia/reoxygenation), and H4 group (with BH from hypoxia-preconditioned mice and hypoxia/reoxygenation). The viability and apoptosis of the cells in the 5 groups were observed by MTT assay and flow cytomertry, respectively. Results The cell viability was significantly higher in the normal control group than in H<,4>R<,48> group. In H0, H1, and H4 groups, the cell viability increased significantly as compared with that in H<,4>R<,48> group, and the cells in H4 group showed the highest viability. Apoptotie cells were scarcely observed in the normal control group, but were numerous in H<,4>R<,48> group. Compared with H<,4>R<,48> group, H0, H1, and H4 groups showed obviously reduced apoptotic cells, and the reduction was the most conspicuous in H4 group. Conclusion BH extracted from hypoxia-preconditioned mice may offer protection against hypoxic injury of rat embryonic hippocampal neurons challenged with hypoxia-reoxygenation by promoting the cell viability and decreasing cell apoptosis.
