Changes in gamma-aminobutyric acid type A receptor α1 subunit following convulsion induced by magnesium-free medium in developing cortical neurons
10.3760/cma.j.issn.1671-8925.2009.11.003
- VernacularTitle:无镁诱导惊厥后发育中皮层神经元γ-氨基丁酸A受体α1亚单位的变化
- Author:
Jian LI
1
;
Ding-An MAO
;
Tao BO
;
Xing-Faug LI
;
Lu YI
;
Zhu-Wen YI
Author Information
1. 中南大学湘雅二医院
- Keywords:
Convulsion;
Type A gamma-aminobutyric acid receptor;
α1 subunit;
Neurons;
Development
- From:
Chinese Journal of Neuromedicine
2009;8(11):1090-1093
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the expression of gamma-aminobutyrie acid type A receptor (GABAAR) α1 subunit following convulsion induced by magnesium-flee medium in developing cortical neurons. Methods Rat embryo cortical neurons cultured for 6 days were cultured for 3 h in normal or magnesium-flee extracellular fluid, followed by further culture in DMEM. At 1, 7 and 12 days after magnesium-free treatment, RT-PCR, immunocytochemical staining, and flow cytometry were performed to detect the expression of GABAAR α1 subunit. Results Compared with that in the control group, GABAAR α1-positive cells in the magnesium-free group decreased significantly at 12 days (P<0.05). Immunoeytochemical staining showed a significant decrease of accumulated optical density (AOD) of GABAAR α1 subunit in the magnesium-free group at 7 and 12 days as compared with that in the control group (P<0.05); the mRNA expression of GABAAR α1 subunit in the magnesium-free group decreased significantly at 1 day compared to that in the control group (P<0.05). Conclusion The expression of GABAAR α1 subunit is altered following convulsion induced by magnesium-free medium in developing cortical neurons.
