Effect of berberine on microglia activation in rats with Alzheimer's disease
10.3760/cma.j.issn.1671-8925.2009.09.010
- VernacularTitle:小檗碱对阿尔茨海默病大鼠模型小胶质细胞活化的影响
- Author:
Fei-Qi ZHU
1
;
Guo-Hou HE
;
Zhi-Jian LIANG
;
Yong-An SUN
;
Wen-Zheng CHU
;
Cai-Yun QIAN
Author Information
1. 郧阳医学院附属太和医院
- Keywords:
Alzheimer's disease;
Berberine;
Microglia;
PPARγ
- From:
Chinese Journal of Neuromedicine
2009;8(9):902-906
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the effect ofberberine chloride on microglia activation and expression of peroxisome prolifcrator-activated receptor gamma(PPAR γ)in a rat model of Alzheimer's disease(AD).Methods Eighteen adult male Sprague-Dawley rats were randomized into normal control,β-amyloid 4o(Aβ40)and Aβ40+berberine chloride groups(n=6).Rat models of AD were established by injection of Aβ40(5 μg)into the bilateral hippocampus,and in Aβ40+berberine chloride group,berberine chloride(50 mg/kg)was given intragastrically once daily for 14 days.Immunohistochemistry,real-time polymerase chain reaction and Western blotting were used to detect the expressions of CD11b and peroxisome proliferator-activated receptor γ(PPAR γ)in the rats.Results The numbers of CD11b positive cells were 74.0±13.4,121.5±19.9 in the Aβ40 and Aβ40+berberine chloride groups,respectively,and the relative copy numbers of CD11b mRNA 4.08±2.43,5.52±1.83,which were significantly increased compared those with normal control group.The numbers of PPARγ positive cells were 42.5+5.6,31.7±8.7,the relative copy numbers of PPARγ mRNA 16.3±13.5,10.8±7.5,and the relative expression of PPARγ protein 0.18±0.08,0.09±0.05,which were significantly decreased compared those with normal control group(93.2±11.3,40.6±17.1,0.31±0.11).Berberine further increased CD11b expression and decreased PPARγ expression in the hippocampus.Conclusion Berberine can increase microglia activation in AD rats by inhibiting the expression of PPARγ.
