NADPH oxidase activation is essential for HSV-1-induced MMP-9 expression in murine microgli
10.3760/cma.j.issn.1671-8925.2009.09.009
- VernacularTitle:NADPH氧化酶对HSV-1感染的小胶质细胞MMP-9激活的影响
- Author:
Yu ZHOU
1
;
Xuan LIU
;
Yuan-Jin GUO
;
Yuan-Wu MEI
Author Information
1. 武汉大学人民医院
- Keywords:
Herpes simplex virus 1;
Microglia;
NADPH oxidase;
Reactive oxygen species;
Matrix metalloproteinase-9
- From:
Chinese Journal of Neuromedicine
2009;8(9):898-901
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the role of NADPH oxidase in Herpes simplex virus 1 (HSV-1)-induced matrix metalloproteinase 9(MMP-9)expression in murine microglial BV2 cells.Methods BV2 cells induced by HSV-1 were divided into normal control group,HSV-1 infection group,and two apocynin treatment groups(in which apocynin was administered at 0.5 and 1.0 mmol/L after HSV-1 infection).MMP-9 gelatinolytic activity in the supematants of the cell cultures Was assessed by zymography.Semi-quantitative reverse transcription polymerase chain reaction(RT-PCR)was used to detect the mRNA expressions of NADPH oxidase subunit p47phox and matrix metalloproteinase-9 (MMP-9),and the intracellular reactive oxygen species(ROS)levels were measured by dihydroethidium staining(DHE).Results Compared to the normal control group,HSV-1 infection of the cells resulted in significantly up-regulated mRNA expression of NADPH oxidase subunit p47phox and MMP-9,and also in a 2-fold increase in the intracellular ROS level(P＜0.05).These changes were attenuated by the application of apocynin,but the mRNA expressions of p47phox and MMP-9 and ROS level still remained significantly higher than those in the normal control group(P＜0.05).Conclusion HSV-1 may induce MMP-9 activation through the generation of NADPH oxidase-dependent ROS in murine microglia.
