Expression of NDRG1 gene in human glioma
10.3760/cma.j.issn.1671-8925.2009.09.005
- VernacularTitle:NDRG1基因在胶质瘤中的表达及意义
- Author:
Bo-Qian SUN
1
;
Da-Ke CHU
;
Wei LI
;
Wei LIN
;
Dun WEI
Author Information
1. 北华大学医学院附属医院
- Keywords:
NDRG1;
Glioma;
Real-time polymerase chain reaction;
Western blotting
- From:
Chinese Journal of Neuromedicine
2009;8(9):881-883,888
- CountryChina
- Language:Chinese
-
Abstract:
Objective To analyze the expression of NDRG1 gene in human glioma tissues.Methods Eighty-three samples of glioma tissues(including 19 grade Ⅰ,22 grade Ⅱ,25 grade Ⅲ,and 17 grade Ⅳ gliomas)and 12 normal brain tissues were obtained from patients undergoing surgeries between February,2006 and June,2007.The mRNA and protein expression of NDRG1 in the tissues was detected using real-time PCR and Western blotting,respectively.Results NDRG1 expressions in the gliomas at both the mRNA and protein levels in comparison with those in normal brain tissues.PCR showed that the NDRG1 expression decreased as the upgrading of tumor from Ⅰto Ⅳ,except grade Ⅱ and grade Ⅲ.(P＜0.05)Conclusion NDRG1 expression is reduced in glioma tissues in close correlation to the pathological grade,suggesting that NDRG1 may play an important role in the occurrence and progression of gliomas.
