Effect of hypoxia on the expressions of hypoxia-inducible factor-1α,glucocorticoid receptor and opiomelanocortin genes in AtT-20 cells

VernacularTitle:低氧对AtT-20细胞HIF-1α、GR和POMC基因表达的影响及意义
Author: Chen-Ran ZHANG ¹ ; Qing-Fang SUN ; Liu-Guan BIAN ; Wei-Guo ZHAO ; Jian-Kang SHEN ; Na LI ; Xiao-Ying LI ; Guang NING
Author Information

1. 第二军医大学附属长征医院
Keywords: Hypoxia-inducible factor-1α; Glucocorticoid receptor; Opiomelanocortin
From: Chinese Journal of Neuromedicine 2009;8(9):873-876
CountryChina
Language:Chinese
Abstract: Objective To investigate the effect of CoCl2-induced hypoxia on the expressions of hypoxia-inducible factor-1α(HIF-lα),glucocorticoid receptor(GR)and opiomelanocortin(POMC)in AtT-20 cells.Methods AtT-20 cells were exposed to different concentrations(25,50,100,and 200 μmol/L)of CoCl2 to induce hypoxia.MTT assay was employed to assess the changes in the cell proliferation after the exposure,and real-time PCR and Western blotting were performed to detect the expressions of HIF-1α,GR and POMC genes at both the mRNA and protein levels.Results Compared with the cells in normoxic conditions,the AtT-20 cells exposed to CoCl2 treatment at 25,50,and 100 μmol/L for 24 and 48 h showed obviously enhanced cell proliferation.CoCl2 significantly increased the mRNA and protein expressions of HIF-1α,GR and POMC genes in a dose-dependent manner(P＜0.05).Conclusion CoCl2-induced hypoxia up-regulates the mRNA and protein expressions of HIF-1α,GR and POMC genes in AtT-20 cells.