Bone marrow stromal cell transplantation for treatment of Parkinson's disease in rhesus monkeys

VernacularTitle:骨髓基质细胞移植治疗帕金森病河猴的实验研究
Author: Li ZHANG ¹ ; Bin XU ; Cheng-Chuan JIANG
Author Information

1. 郧阳医学院附属太和医院
Keywords: Parkinson's disease; Bone marrow stromal cells; Transplantation
From: Chinese Journal of Neuromedicine 2009;8(8):794-797
CountryChina
Language:Chinese
Abstract: Objective To evaluate the therapeutic effect of bone marrow stromal cell transplantation on Parkinson's disease (PD) in rhesus monkeys. Methods Experimental PD was induced in rhesus monkeys by stereotatic surgery to destroy the unilateral substantia nigra. Human bone marrow stromal cells were injected stereotaxically into the 9 target areas in the brain of the monkeys, after which behavioral test was performed and the alteration in the dopamine transporter (DAT) in the substantia nigra was examined using single-photon emission computerized tomography (SPECT). Immunofluorescence assay was employed to examine the TH-and BrdU-positive cells in the injured and contralateral substantia nigra. Results All the monkeys showed progressive deterioration of the PD symptoms after the cell transplantation. SPECT revealed no significant changes in DAT in the bilateral substantia nigra, but immunoflurescence assay showed that a proportion as high as 25% of the transplanted BMSCs differentiated into dopaminergic neurons. Conclusion Human BMSCs can not serve as the donor cells for treatment of PD in rhesus monkeys.