Expressions of ATM, ATR, Chk1 and Chk2 genes in human glioma

VernacularTitle:人脑胶质瘤中ATM、ATR、Chk1和Chk2基因表达研究
Author: Xue-Mei ZHOU ¹ ; Jian QIAO ; Rao WANG ; Jian-Ping HUANG ; Hui-Bo WANG ; Yu-You QIU ; Yong-Zhen CHEN
Author Information

1. 苏州大学医学部
Keywords: Glioma; DNA damage; Cell cycle checkpoint; ATM; ATR; Chk1; Chk2
From: Chinese Journal of Neuromedicine 2009;8(7):653-657
CountryChina
Language:Chinese
Abstract: Objective To detect the expressions of DNA damage checkpoint genes including A TR, A TM, Chk1 and Chk2 in human primary gliomns and explore their relations with tumor progression. Methods SYBRTM Green real-time quantitative PCR was performed to detect the expressions of ATR, A TM, Chk1 and Chk2 genes in 35 cases of primary gliomas and 10 of normal brain tissues. Results In glioma tissues of various pathological grades, the expressions of the target genes, with the exception of A TM gene, were significantly increased as compared to those in normal brain tissues (P<0.05). Chk1 gene expression was significantly higher in grade Ⅳ than in grade Ⅱ and Ⅲ gliomas (P<0.05), but no significant differences were found in A TR or Chk2 gene expression between grade Ⅱ, Ⅲ and Ⅳ gliomas (P>0.05). Conclusion The up-regulation of ATR, Chk1 and Chk2 genes in primary glioma suggests their association with the pathogenesis of glioma. Chk1 expression may indicate the malignancy of glioma and help evaluate the pathological grade of glioma.