Genetic analysis of adult human bone marrow-derived neural stem cells with strong migration potential
10.3760/cma.j.issn.1671-8925.2009.07.001
- VernacularTitle:成人骨髓源性神经干细胞高迁移能力的基因分析
- Author:
Ru-Sen ZHU
1
;
Ru-Xiang XU
;
Xiao-Dan JIANG
;
Ying-Qian CAI
;
Yuxi ZOU
;
Mou-Xuan DU
Author Information
1. 广东医学院附属医院
- Keywords:
Neural stem cells;
Invasion;
Metastasis;
Microarray;
Gene expression
- From:
Chinese Journal of Neuromedicine
2009;8(7):649-652
- CountryChina
- Language:Chinese
-
Abstract:
Objective To analyze the gene expression profiles in relation to the migration ability of adult human bone marrow-derived neural stem cells (Md-NSCs), and identify the genetic basis of the high migration potential of Md-NSCs in the central nervous system (CNS). Methods Adult human bone marrow stromal celIs(BMSCs) obtained from adult healthy volunteers were induced to differentiate into Md-NSCs in vitro, and the expressions of the genes related to cell invasiveness and metastasis were investigated by microarray analysis. Quantitative real-time PCR (RT-PCR) was used to verify the microarray results. Results The results of microarray analysis revealed significant overexpressions of the genes MMP1, MMP2, MMP17, ITGA3, RhoB, RhoC and RhoD in the Md-NSCs as compared with the expressions in fresh normal human adult bone marrow cells depleted of red blood cells. Quantitative RT-PCR verified the overexpression ofMMP2 gene by 2.84×100 folds, ITGA3 gene by 2.22×102folds, and RhoC gene by 4.92×100 folds. Conclusion The high migration potential of the Md-NSCs in the CNS is probably associated with the overexpression of the genes that promote cell invasiveness and metastasis. These overexpressed genes are also important oncogenes, and therefore the tumorigenicity of the Md-NSCs warrants further investigation.
