Feasibility of local LINGO-1 polyclonal antibody treatment for spinal cord injury in adult rats
10.3760/cma.j.issn.1671-8925.2009.05.011
- VernacularTitle:LINGO-1多克隆抗体对大鼠脊髓损伤模型干预的可行性分析
- Author:
Jun L(U)
1
;
Ru-Xiang XU
;
Zhi-Qiang FA
;
Xiao-Dan JIANG
;
Xin LU
;
Yi-Quan KE
;
Ying-Qian CAI
;
Mou-Xuan DU
;
Yu-Xi ZOU
;
Ling-Sha QIN
Author Information
1. 南方医科大学珠江医院
- Keywords:
Central nervous system;
Spinal cord injury;
LINGO-1;
Polyclonal antibody
- From:
Chinese Journal of Neuromedicine
2009;8(5):476-478,483
- CountryChina
- Language:Chinese
-
Abstract:
Objective To analyze the feasibility of local LINGO-1 polyclonal antibody administration for treatment of spinal cord injury in adult rats. Methods Twenty-four adult female SD rats were randomized into sham-operated group, rabbit IgG group and LINGO-1 antibody group. In the latter two groups, partial transaction of the T9 segment of the spinal cord was performed to completely sever the dorsal eorticospinal tract, followed immediately by administration of rabbit IgG and anti-LINGO polyclonal antibody via a mini-osmotic pump, respectively. At 3 and 28 days after the operation, the T8～10 segments of the spinal cord were harvested to prepare cryosections, and immunofluorescence staining was used to analyze the penetration of LINGO-1 polyclonal antibody into the spinal cord tissue and its specific binding to LINGO-1 molecules. Results In LINGO-1 antibody group, the presence of rabbit antibodies was detected at the injured sites of the spinal cord at 3 and 28 days after the operation. The mean immunofluorescence density was significantly lower in L1NGO-1 antibody group than in rabbit IgG group at 3 days after the operation (P<0.05). In rabbit IgG group, the mean immunofluorescence density for LINGO-1 in the crysections pre-treated with LINGO-1 polyclonal antibody was significantly lower than that in sections pre-treated with rabbit IgG(P<0.05). Conclusion Locally administered LINGO-1 polyclonal antibody can penetrate into the injured sites in the spinal cord in a wide time window and recognizes LINGO-1 molecule specifically, suggesting the feasibility of passive immunotherapy for spinal cord injury.
