Sevoflurane promotes proliferation of adult rat neural stem cells in vitro
10.3760/cma.j.issn.1671-8925.2009.05.010
- VernacularTitle:七氟烷促进成年大鼠海马神经干细胞增殖
- Author:
Ya-Ting ZHANG
1
;
Miao-Ning GU
Author Information
1. 南方医科大学南方医院
- Keywords:
Sevoflurane;
Neural stem cells;
Proliferation;
cAMP response element bindingprotein;
Phosphorylation
- From:
Chinese Journal of Neuromedicine
2009;8(5):472-475
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the effect of sevoflurane in promoting the proliferation of adult rat neural stem cells (NSCs) in vitro and explore its possible molecular mechanisms. Methods Adult rat NSCs in routine cell culture were identified with immunofluorescence labeling using nestin antibody. After a 2-h culture, the NSCs were placed in an airtight and temperature-controlled cell culture chamber for a 2-h exposure to sevoflurane at the concentrations of 1.3%, 1.9%, 2.7%, and 3.3%. The cells were then routinely cultured in the presence of 5% CO2 for 24 h. DAPI staining was used to assess the changes in cell proliferation and apoptosis, and Western blot performed to detect the phosphorylation level of cAMP response clement binding protein (CREB). Results Compared with the control cells, the NSCs exposed to 1.9%, 2.7%, and 3.3% sevoflurane showed significantly increased cell number after the 24-h incubation, and the increment increased significantly with the concentration of sevoflurane (P<0. 05). Western blotting detected the presence of CREB and phospho-CREB expressions in both the control cells and the cells exposed to 1.3% and 1.9% sevoflurane, and the expression levels were the highest in cells exposed to 1.9% sevoflurane (P<0.05). Conclusion Sevoflurane can promote the proliferation of adult rat NSCs in vitro possibly by enhancing the phosphorylation of CREB.
