Reliability of Schwann cell acquisition by inducing the differentiation of bone marrow stromal cells in vitro
10.3760/cma.j.issn.1671-8925.2009.05.009
- VernacularTitle:体外诱导获得雪旺细胞的可靠性研究
- Author:
Zhong-Jie YAN
1
;
Ru-Xiang XU
;
Hong-Tian ZHANG
;
Xiao-Dan JIANG
;
Jian-Hao LIN
;
Xu MA
;
Xi-Feng LI
;
Xin-De ZHAO
Author Information
1. 南方医科大学珠江医院
- Keywords:
Schwann cells;
Cell differentiation;
Bone marrow stromal cells
- From:
Chinese Journal of Neuromedicine
2009;8(5):468-471,475
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the reliability of acquiring Schwann cells from bone marrow stromal cells (BMSCs) by inducing their differentiation in vitro. Methods BMSCs isolated from the tibial and femural bone marrow of SD rats by adherent culture were passaged and induced with beta-mercaptoethanol followed by retinoic acid, forskolin, basic fibroblast growth factor (bFGF), platelet-derived growth factor (PDGF) and heregulin. Immunocytochemistry was performed to identify the expression of the Schwann cell markers P75, S100 and glial fibrillary acidic portein (GFAP), and fluorescent real-time quantitative RT-PCR was used to detect the mRNA expressions of P75, S100 and CD104 in the induced BMSCs. Results The induced BMSCs exhibited morphological changes into cells resembling primary cultured Schwann ceils, and expressed P75, S100 and GFAP proteins, as shown by immunofluorescent staining, at the levels similar to those in Schwann cells. The induced cells, however, remained negative for P75 mRNA expression as demonstrated by RT-PCR. Conclusion The BMSCs can be induced to partially acquire the phenotypic features of Schwann cells, suggesting the strong plasticity of the BMSCs. The protocol of pre-induction followed by induction with an array of agents still needs further modification to induce the in vitro differentiation of BMSCs into Schwann-like cells.
