Extracellular signal-regulated kinases1/2 is involved in the inhibition of radiation-induced apoptosis of C17.2 neural stem cells by exogenous basic fibroblast growth factor
10.3760/cma.j.issn.1671-8925.2009.05.008
- VernacularTitle:外源性bFGF抑制辐射诱导的C17.2神经干细胞凋亡的实验研究
- Author:
Hai-Hong ZHOU
1
;
Yun-Lin LIU
;
Song-Hua XIAO
;
Jun LIU
;
Rui-Yan LU
;
Bin ZHAO
Author Information
1. 广东医学院附属医院
- Keywords:
Basic fibroblast growth factor;
Extracellular signal-regulated kinases1/2;
Apoptosis;
Neural stem ceils
- From:
Chinese Journal of Neuromedicine
2009;8(5):464-467
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the effects of exogenous basic fibroblast growth factor (bFGF) on radiation-induced apoptosis of C 17.2 neural stem cells (NSCs) and explore the role of ERK1/2 in this process. Methods C17.2 NSCs were subjected to irradiation exposure generated by a linear accelerator followed by treatment with different concentrations of bFGF 5 min after the exposure. The cell viability after the treatments was assessed using MTT assay, and the effects of exogenous bFGF and U0126 on radiation-induced apoptosis of the cells were analyzed with flow cytometry. Results The apoptotic rate of the irradiated cells decreased from (12.78±1.04)% to (4.83±0.3 I)% as the concentration ofbFGF increased from 0 to 100 ng/mL. The apoptotic rates of the cells after treatment with U0126 and DMSO were (8.42±0.71)% and (4.71±0.42)%, respectively, showing a significant difference (P<0.05). Conehlsion Exogenous bFGF offers protection of C17.2 NSCs against radiation-induced apoptosis, and extracellular signal-regulated kinases 1/2 (ERK1/2) plays a role in this protective effect.
