Comparison of the differential potentials of bone marrow and adipose-derived stromal cells into neuron-like cells in vitro
10.3760/cma.j.issn.1671-8925.2009.05.003
- VernacularTitle:骨髓源及脂肪源干细胞向神经元样细胞诱导分化潜力的比较研究
- Author:
Xu MA
1
;
Xiao-Dan JIANG
;
Hong-Tian ZHANG
;
Jian-Hao LIN
;
Zhong-Jie YAN
;
Ling-Sha QIN
Author Information
1. 南方医科大学珠江医院
- Keywords:
Bone marrow-derived stromal cells;
Adipose-derived stromal cells;
Neuron-likecells;
Cell differentiation
- From:
Chinese Journal of Neuromedicine
2009;8(5):441-445
- CountryChina
- Language:Chinese
-
Abstract:
Objective To compare the differentiation potentials of bone marrow-derived stromal cells (BMSCs) and adipose-derived stromal cells (ADSCs) into neuron-like cells in vitro. Methods The fifth-passage of cultured adult SD rat BMSCs and ADSCs were induced with 10 ng/mL epidermal growth factor (EGF) and 20 ng/mL basic fibroblast growth factor (bFGF). After induction for 6, 12, 24, 72 h and 1 and 2 weeks, the cells were harvested to examine the expressions of the neural markers nestin and β-tubulin Ⅲ using immunohistoChEnistry and Western blot. Results Both the BMSCs and ADSCs underwent morphological changes into neuron-like cells and expressed the neuron-specific markers after the induction. The two cells exhibited significantly different positivity rates for nestin and β-tubulin Ⅲ after the induction (P<0.05). The ADSCs exhibited stronger ability than BMSCs to differentiate into neuron-like cells shown by greater nestin and β-tubulin Ⅲ positivity rates after the induction. Conclusion ADSCs possess stronger capacity of induced differentiation into neuron-like ceils than BMSCs in in vitro culture.
