Establishment of synapses between rat cortical neurons and Neuron-like cells derived from bone marrow stromal cells in vitro
10.3760/cma.j.issn.1671-8925.2009.04.002
- VernacularTitle:骨髓基质细胞分化为神经元样细胞后与皮质神经元间突触的建立
- Author:
Hui ZHOU
1
;
Xiao-Dan JIANG
;
Yi-Zhao CHEN
;
Li YANG
;
Zhi-Qiang FA
;
Yu-Xi ZOU
Author Information
1. 广东神经外科研究所
- Keywords:
Synapses;
Bone marrow stromal cells;
Neurons;
Co-culture;
FM1-43
- From:
Chinese Journal of Neuromedicine
2009;8(4):331-334,339
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the establishment of synapses between the cortical neurons and the neuron-like cells difierentiated from the marrow stromal cells(BMSCs)in a simulated transplantation system in vitro.Methods The BMSCs from green fluorescent protein(GFP)transgenic mice(GFP-GM-BMSCs) were isolated, cultured and purified in vitro.The third passage of GFP-GM-BMSCs were co-cultured with primary cultured cortical neurons and gliai cells in a simulated transplantation system in serum-free medium conmining 2%B27 supplemented with 20 ng/mL basic fibroblast growth factor(bFGF)and 20 ng/mL epidermal growth factor(EGF).On day 10 of the co-culture,FM1-43,a fluorescent dye specific to active synaptic vesicles,was used to observe synapses formation between the cells under fluorescence microscope. Results The GFP.GM-BMSCsco-cultured with the neural cells in the Serum-free medium containing bFGF and EGF differentiated into neuron-like cells 7 days after the co-culture.On day 10 ofthe co-culture,FM1-43 dye-positive synaptic vesicles were foundin the cell culture,locating mostly in the cell body,processes and terminal sffuctures ofthe neuron-like cells. Conclusions The neuron-like cells derived from GFP-GM-BMSCs can form synapses with the coRical neurons in the simulated cell transplantation system in vitro.
