Phenotypic and functional analysis of bovine gammadelta lymphocytes..
- Author:
Yong Ho PARK
1
;
Han Sang YOO
;
Jang Won YOON
;
Soo Jin YANG
;
Jong Sam AN
;
W C DAVIS
Author Information
1. Department of Microbiology and Infectious Diseases, College of Veterinary Medicine and School of Agricultural Biotechnology, Seoul National University, Suwon 441-744, Korea. yhp@plaza.snu.ac.kr
- Publication Type:Original Article ; Research Support, Non-U.S. Gov't
- Keywords:
WC1+ gammadelta T cells;
WC1- gammadelta T cells;
T cell receptor;
MHC restricted immune responses
- MeSH:
Animals;
Base Sequence;
Cattle;
Concanavalin A;
Cytokines/genetics/immunology;
DNA Primers;
Immunophenotyping;
Lymph Nodes/immunology;
Lymphocyte Activation;
Receptors, Antigen, T-Cell, gamma-delta/*immunology;
Reverse Transcriptase Polymerase Chain Reaction;
T-Lymphocytes/classification/*immunology
- From:Journal of Veterinary Science
2000;1(1):39-48
- CountryRepublic of Korea
- Language:English
-
Abstract:
The studies have provided the first comprehensive comparison of the factors regulating activation and proliferation of WC1+ and WC1- gammadelta T cells. The investigation has shown that accessory molecules essential for activation and function of WC1+ and WC1- gammadelta T cells and the sources and roles of cytokines in activation of gammadelta T cells through the T cell receptor (TCR). The study has also shown that the role of cytokines in activation and function of gammadelta T cells activated indirectly through cytokines secreted by ab T cells, accessory cells and antigen presenting cells (APC). Cytokines were differentially produced by subpopulations of gammadelta T cells under different conditions of activation. The investigation obtained in this study has revealed that factors account for activation and proliferation of gammadelta T cells in cultures designed to study MHC-restricted responses to antigens. Evidence obtained here has shown there is biological relevance to activation under these culture conditions that points to potential regulatory and effector functions of gammadelta T cells. The investigations have also provided the information needed to begin identifying and characterizing antigens recognized by the TCR repertoires of WC1+ and WC1- gammadelta T cells. Finally, the investigations have provided the information needed to begin analysis of the mechanisms by which gammadelta T cells modulate MHC restricted immune responses to pathogens and derived vaccines.
