Effect of electro-acupuncture on the expression of NCAM and NF-kB in SamP8 mouse hippocampus
10.3760/cma.j.issn.1671-8925.2009.03.015
- VernacularTitle:电针对SAMP8小鼠海马NCAM和NF-κB表达的影响
- Author:
Sheng-Feng LU
1
;
Yong TANG
;
Hai-Yan YIN
;
Ling-Ling GUO
;
Xiao-Hua PENG
;
Shu-Guang YU
Author Information
1. 成都中医药大学
- Keywords:
Electro-acupuncture therapy;
Alzheimers' disease;
Hippocampus;
Neural cell adhesion molecule;
Nuclear factor kappa B
- From:
Chinese Journal of Neuromedicine
2009;8(3):266-269
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of electro-acupuncture (EA) therapy on the syuaptic plasticity of hippocampal neurons in senescence-accelerated mouse (SAMP8) by examining the changes in neural cell adhesion molecule (NCAM) and nuclear factor-κB (NF-κB) expression, and explore the mechanism behind the therapeutic effect of EA on Alzheimer's disease (AD) in view of neural cell adhesion. Methods Twenty-four SAMP8 mice as the animal model of AD were randomized equally into the model group and EA treatment group, with 12 senescence-accelerated resistant mice (SAMR1) mice as the blank control group. EA on Baihui (Du20) and Yongquan (Kid1) was administered once daily for 21 consecutive days in mice in the EA group. The expressions of NCAM and NF-κB mRNA and proteins in the hippocampal neurons were detected by immunohistochemistry and in situ hybridization. Results Compared with the model group, the mice in EA group showed significantly increased expression of NCAM and NF-κB mRNA and proteins (P<0.05). Conclusion EA can increase the expression of NCAM and NF-κB in mouse hippocampal neurons. EA can promote the cell adhesion and synapse plasticity of the neurons possibly by upregulating NF-κB expression to induce increased NCAM production.
