Relation between lymphocyte subpopulations of peripheral blood and immune responses of modified live hog cholera virus vaccine in pigs treated with an ionized alkali mineral complex.
- Author:
Bong Kyun PARK
;
Yong Ho PARK
;
Kyung Suk SEO
- Publication Type:Original Article ; Comparative Study ; Research Support, Non-U.S. Gov't
- Keywords:
Ionized alkali mineral complex;
lymphocyte sub-populations;
attenuated hog cholera virus vaccine
- MeSH:
Animal Feed;
Animals;
Antibodies, Monoclonal/*blood/isolation & purification;
Antigens, CD2/blood;
B-Lymphocytes/immunology;
CD4-Positive T-Lymphocytes/immunology;
CD8-Positive T-Lymphocytes/immunology;
Classical Swine Fever/*immunology;
Classical swine fever virus/*immunology;
Dietary Supplements;
Ions;
Lymphocyte Subsets/*immunology;
*Minerals;
Swine;
Vaccines, Attenuated/*administration & dosage;
Viral Vaccines/*administration & dosage
- From:Journal of Veterinary Science
2000;1(1):49-52
- CountryRepublic of Korea
- Language:English
-
Abstract:
Thirty-nine healthy pigs (28-32 days old) were purchased from a commercial swine farm and housed at swine pens of the College. The animals were vaccinated intramuscularly (1 ml) with an attenuated live hog cholera virus (HCV, LOM strain) and then boostered at 5 weeks after the first vaccination. The animals were divided into 4 experimental groups: 0.05% (w/w) PowerFeel-supplemented diet (T-1, n = 10); 3% (w/w) SuperFeed-supplemented diet (T-2, n = 10); diluted PowerFeel solution (1 : 500, v/v) as drinking water (T-3, n=9); control (n=10). PowerFeel is an original form of ionized alkali mineral complex (IAMC) and SuperFeed is a commercial product of IAMC. The subpopulation of lymphocyte in blood was assayed by a flow cytometry and HCV-specific antibody was determined by an indirect immunofluorescence assay. In IMAC-treated groups, the proportions of subpopulation expressing MHC-class II, CD2+, CD4+, CD8+, and surface IgM+ B lymphocytes were significantly decreased at 5-weeks after the first vaccination. Significant decreases were also observed in the proportions of MHC-class II, CD2+ and CD8+ lymphocyte at 3-weeks after the booster injection. The humoral immune responses in T-1 and T-2 groups were greater than those in T-3 or control group. These results suggest that IAMC-supplemented diets may have an HCV-specific immunostimulatory effect in pigs.
