Hippocampal Zinc transporter 3 mRNA expression and metabolic changes defined by 1H-MRS in weaning rats following heat stress and febrile convulsion
10.3760/cma.j.issn.1671-8925.2009.03.011
- VernacularTitle:急性热应激与热惊厥幼龄大鼠海马ZnT3 mRNA表达和代谢变化的1H-MRS波谱研究
- Author:
Hong NI
1
;
Da-Qing CHEN
;
Wei-Liang ZHENG
;
Quan-Xiang SHUI
Author Information
1. 苏州大学附属儿童医院
- Keywords:
Febrile seizures;
Nuclear magnetic resonance;
Spectroscopy,Zinc transporter 3
- From:
Chinese Journal of Neuromedicine
2009;8(3):250-253,257
- CountryChina
- Language:Chinese
-
Abstract:
Objective To asses the value of proton magnetic resonance spectroscopy (1H-MRS) combined with neuropathological findings in early detection of metabolic abnormities and damages of the brain neurons following heat stress (HS) and febrile convulsion (FC). Methods Febrile convulsion models were established in weaning rats (21 days old) by means of hot water bath. -1H-MRS was performed to measure the changes in N-acetylaspartate (NAA), choline (cho), lactate (Lac) and creatine (Cr) contents in the brain tissue following HS or FC, and in sire hybridization was used to detect Zinc transporter 3 (ZnT3) mRNA expression in the hippocampus. Results In the control group, HS group and FC group, the NAA/Cr ratio (1.5±0.42, 1.57±0.59, and 1.61±0.37, respectively) and Cho/Cr ratios showed no significant differences, but a significant increase in Lac/Cr ratio was observed in FC group. ZnT3 3 mRNA expression was detected in the dentate gyms of the rats following the onset of FC. Conclusions As Lac increase is a putative marker of seizure-induced neuronal damage, and ZnT3 is associated with mossy fiber sprouting in the hippocampus, our results suggest that even a single temporary FC may result in marked changes in neuronal metabolism and cause subtle brain injury.
