Comparison of the effect of adult neural stem cells derived from different origins in treatment of rat spinal cord injury
10.3760/cma.j.issn.1671-8925.2009.03.010
- VernacularTitle:不同来源成体神经干细胞促进大鼠脊髓损伤功能修复的比较研究
- Author:
Ying-Qian CAI
1
;
Hong-Tian ZHANG
;
Xiao-Dan JIANG
;
Ru-Xiang XU
;
Lin-Lang GUO
Author Information
1. 南方医科大学珠江医院
- Keywords:
Spinal cord injury;
Cell transplantation;
Neurospheres
- From:
Chinese Journal of Neuromedicine
2009;8(3):246-249
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate the therapeutic effects of adult neural stem cells derived from the brain, adipose tissue and bone marrow on spinal cord injuries in adult rats. Methods The brain subventricular zone (SVZ), adipose tissue and bone marrow from the same rat were obtained to induce the neural stem cells (NSCs). In 72 SD rats with spinal contusive injury, the NSCs from the 3 origins were grafted into the injured spinal cord one week after the injury, with 24 rats as the saline control group and another 24 as the sham-operated group. The locomotor recovery of the rats was evaluated according to the BBB scores, and the cell survival, distribution, migration and differentiation in the injured spinal cord were evaluated by immunohistochemistry. Results Compared with the rats in sham-operated and saline groups, the rats receiving transplantation of NSCs of different origins all showed significantly increased BBB scores. At 9 weeks after the transplantation, the rats receiving brain SVZ-derived NSCs (SVZ-NSs) exhibited significantly improved locomotor function compared with those grafted with the other two NSCs (P<0.05). The SVZ-NSs showed significantly higher Brdu/nestin+cell percentage than bone marrow-derived NSCs(BM-NSs) and adipose-derived NSCs(AD-NSs) at 4 weeks after grafting, but till 8 week after the grafting, only a few positive cells were found in the injured spinal cord in the 3 groups, without significant difference between them (P>0.05). Conclusion Grafting of the NSCs derived form the brain SVZ, adipose tissue and bone marrow all help improve the locomotor recovery of the rats following spinal cord injury, and the SVZ-NSs has the most obvious effect. But AD-NSs may seem a better option than those of other origins for repairing the injured spinal cord due to their abundant sources and strong proliferation ability.s
