Genotyping and drug resistance analysis of Candida albicans in the intensive care unit of the neurosurgical department

VernacularTitle:神经外科重症监护室白色念珠菌基因分型和耐药性研究
Author: Jun LONG ¹ ; Meng LAN ; Hui ZHONG ; Chang-Hong JIANG
Author Information

1. 南方医科大学珠江医院
Keywords: Candida albicans; Genotyping; Drug resistance; Intensive care unit of neurosurgical department
From: Chinese Journal of Neuromedicine 2009;8(2):194-196
CountryChina
Language:Chinese
Abstract: Objective To analyze the drug resistance profile of the clinical isolates of Candida albicans and establish a randomly amplified polymorphic DNA (RAPD) assay for Candida albicans. Methods Thirty strains of Candida albicans were collected from the intensive care unit (ICU) of the department ofneurosurgery between May, 2007 and November, 2007. The drug resistance of Candida albicans was analyzed with liquid microdilution method, and the fungal DNA was extracted for genotyping by RAPD assay. Results The sensitivity of Candida albicans strains was 100% to the anti-fungal drug VRC, 100% to AMB (100%), 96.35% to FCZ, 93.0% to 5-FC, and 90.0% to ITR. The 30 Candida albicans strains were genotyped into 27 types with a typing rate of 90.0%. Conclusion AMB and VRC remain the primary options for treatment of Candida albicans infection, and the isolated Candida albicans strains are highly sensitive to FCZ, 5-FC, and ITR. No evidence has been identified to suggest a local outburst ofCandida albicans infection in our hospital between May, 2007 and November, 2007.