Role of hemin-induced heme oxygenuse-1 expression in rat central nervous system after brain trauma
10.3760/cma.j.issn.1671-8925.2009.02.010
- VernacularTitle:血晶素诱导血红素氧合酶-1在脑外伤中的表达及意义
- Author:
Zhong-Hong LIU
1
;
Jia-Quan HE
Author Information
1. 武警重庆市总队医院
- Keywords:
Heine oxygenase-1;
Brain trauma;
Protection
- From:
Chinese Journal of Neuromedicine
2009;8(2):145-147
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the role of heme oxygenase-1 (HO-1) in the central nervous system after brain trauma. Methods Forty-eight adult SD rats were randomized into brain trauma+hemin group, brain trauma+normal saline group and sham-operated group, and in the former two groups, the rats were subjected to fluid percussion injury of the brain followed by intraperitoneal injections of 45 mg/100 mg hemin or saline accordingly. The rats in the sham-operated group received incision of the scalp and drilling on the skull only. The behavioral changes of the rats after the treatments were evaluated by testing the latency of head turning on a ramp. The permeability of the blood brain barrier (BBB) was assessed by measuring the brain tissue content of Evans blue injected into the tail vein, and the expression of HO-1 in the brain tissue was detected using immunohistochemistry with SP method. Results HO-1 expression in the brain tissue of heroin-treated rats (125.52±14.39) was significantly increased as compared with that of normal saline-treated rats (100.63±12.32) and sham-operated rats (79.37±12.89) (P<0.05). The latency of head turning on the ramp was significantly shorter in hemin-treated group (7.38±1.69 s) than in normal saline group (10.01±1.61 s,P<0.05). The permeability of the BBB was markedly decreased in heroin group. Conclusion HO-1 expression in the brain tissue may provide protection of central nervous system after brain trauma.
