Inhibition by Guangxi Selenocosmia huwena toxins on human glioma cell strain U251
10.3760/cma.j.issn.1671-8925.2009.02.008
- VernacularTitle:广西虎纹捕鸟蜘蛛毒素对胶质瘤细胞株U251增殖抑制作用的研究
- Author:
Qin HUANG
1
;
Xin HUANG
;
Mian-Lin LIU
;
Qiu-Hua LIU
;
Yuan-Liang YE
;
Hong-Tao XU
Author Information
1. 广东三九脑科医院
- Keywords:
Glioma;
Guangxi Selenocosmia huwena toxins;
Apoptosis
- From:
Chinese Journal of Neuromedicine
2009;8(2):137-139
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the inhibition by Guangxi Selenocosmia huwena toxins on human glioma cell strain U251 and to interpret its possible mechanisms, with regard to initial clarify its mechanism. Methods Culture medium of Containing 10% fetal bovine serum wet RPMI1640 of U251 cells incubated with culture, MTT was involved indetecting the cytotoxic effects. HE staining and inverted phase contrast microscope was used to detected the change of cell shape and cellular nucleus shape. TUNEL was used to detected apoptosis ofhuman glioma cell strain U251. Results The proliferation of U251 was obviously inhibited by Guangxi Selenocosmia huwena toxins in dose- and time- dependent manner. The inhibitive effect of Guangxi Selenocosmia huwena toxins on U251 was obvious, with the Two half-inhibitory concentration of 82.60 μg/mL and 71.12 μg/mL at 48 h and 72 h respectively, This depressanteffct offer conspicuous dose-effect relationship. We could observesome cell nucleus became to pycnosis and nuclear fragmentation (cell apoptosis). We also could dye apoptosis cell nucleus in brown. Conclusion Guangxi Selenocosmia huwena toxins can inhibit theproliferation of glioma U251 which was implemented through the inducement ofglioma apoptosis.
