Inhibitory effects of Notch receptor blocker MWI67 on the proliferation of U87 glioma cells in vitro

VernacularTitle:Notch受体阻断剂MW167抑制U87胶质瘤细胞增殖的作用研究
Author: Jian-Quan YANG ¹ ; Wei-Cheng YAO ; Shi-Fang LI ; Chang-Qing DING
Author Information

1. 青岛大学医学院附属医院
Keywords: Notch signaling; MW167:Proliferation:Apoptosis; Glioma
From: Chinese Journal of Neuromedicine 2008;7(12):1231-1233,1241
CountryChina
Language:Chinese
Abstract: Objective To observe the effect of Notch receptor blocker MW167 on the proliferation and apoptosis of U87 glioma cell line in vitro. Methods The inhibitory effect of MW167 on the proliferation of U87 cells was investigated by MTT assay, and flow cytometry with Annexin-FITC and PI staining was performed to detect the apoptotic rate of U87 cells after MW167 treatment. Results MTT assay demonstrated an obvious concentration-dependent inhibitory effect of MW167 on the proliferation of U87 cells. Flow cytometry showed that MW167 also concentration-dependently induced U87 cell apoptosis. Conclusion MW167 significantly inhibits the proliferation and induces apoptosis of U87 glioma cells, suggesting the potential therapeutic value of MW167 in the treatment of human glioma. [Key wnrds] Notch signaling;MWI67;Proliferation;Apoptosis;Glioma