Construction of human NR2B eukaryotic expression vector and its expression in CHO cells

VernacularTitle:人NR2B基因真核表达载体的构建及其在CHO细胞中的表达
Author: Yong SHI ¹ ; Hai-Yan ZHU ; Hou-Da LI
Author Information

1. 上海市出入境检验检疫局
Keywords: NR2B gene; CHO cells; Eukaryotic expression vector
From: Chinese Journal of Neuromedicine 2008;7(12):1189-1192
CountryChina
Language:Chinese
Abstract: Objective To clone human NR2B gene, construct its eukaryotic expression vector, and temporarily express it in CHO cells. Methods Human NR2B gene was amplified by RT-PCR and then inserted into eukaryotic vector pcDNA3.1. The recombinant plasmid was transfected into CHO cells. The expression of the target molecule was identified by RT-PCR, Western blotting, indirect immanofluorescent staining and the apoptosis was detected by flow cytometry. Results The NR2B gene was obtained; after transfection, NR2B was successfully expressed in CHO cells, and the expression of NR2B did not induce the apoptosis of CHO cells. Conclusion Human NR2B gene has been successfully cloned and expressed in CHO cells via constructing its eukaryotic expression vector.