Therapeutic effect of Angelica injection in Parkinsonian rats and the mechanisms
10.3760/cma.j.issn.1671-8925.2008.11.017
- VernacularTitle:当归注射液对帕金森病模型大鼠的作用及其机制的研究
- Author:
Teng XIE
1
;
Jie ZHANG
;
Yu-Bo XIONG
;
Zhi-Hua WEN
;
Xian-Hou YUAN
Author Information
1. 武汉大学中南医院
- Keywords:
Parkinson disease;
Angelica injection
- From:
Chinese Journal of Neuromedicine
2008;7(11):1151-1154
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the therapeutic effect of Angelica injection in Parkinsonian rats and explore its possible mechanisms. Methods Rat models of Parkinson disease (PD) were established by 6-OHDA injection into the right substantia nigra pars compacta (SNc) and confirmed by weeldy apomorphine-induced rotation test, and the rats with saline injection served as the saline control group (n=8). After the second apomorphine test, the PD rats received daily intraperitoncal Angelica injection at low (12.5%, n=8) or high dose (25%, n=8), or had only saline injection (PD model group, n=8). Another 8 untreated rats were used as the blank control group. After 4 weeks of treatment, the rats were perfused with paraformaldehyde (4%) and the brains were taken for immunohistochemical detection of TH and CD40 expressions in the SNc using SABC method. Results Compared with the rats in the saline control and low-dose Angelica injection groups, those in the high-dose Angelica injection group showed significantly slowed rotation after a 3-week treatment (P<0.05), with further slowing down at 4 weeks (P<0.01). At the fourth week of treatment with high-dose Angelica injection, a significant increase in the numbers of PH- and CD40-positive neurons was found in the right SNc as compared with those in the low-dose Angelica injection group and the PD model group (P<0.01). No CD40-positive microglia were found in the SNc in the saline control or the blank control groups, and the Angelica-treated groups and the PD model group showed no significant difference in the count of CD40-positive microglia in the SNc. Conclusion Angelica injection produces obvious therapeutic effect in Parkinsonian rats possibly by increasing CD40 expression in the neurons.
