Expression of nuclear factor-kB and COX-2 and their role in experimental allergic encephalomyelitis in rabbits
10.3760/cma.j.issn.1671-8925.2008.11.016
- VernacularTitle:NF-KB p65及环氧化酶-2在实验性变态反应性脑脊髓炎中的表达及其作用机制
- Author:
Cong GAO
1
;
Fu-Hua XIE
;
Jian-Rui YIN
;
Jie YANG
;
Xin FENG
;
Peng-Cheng MA
Author Information
1. 广州医学院附属第二医院
- Keywords:
Experimental allergic encephalomyelitis;
Nucleus transfer factor,Cyclooxygenase-2
- From:
Chinese Journal of Neuromedicine
2008;7(11):1147-1150
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the expression of nuclear factor-kB (NF-KB) and COX-2 and their relationship in experimental allergic encephalomyelitis (EAE), and investigate their role in the pathogenesis of EAE. Methods All rabbits were classified three groups in according to making models by different immunogens: group A is immuned without immunogen, group B is immuned with R-SCH, group C is immunod with C-SCH. Immunofluorescence and immunohistochemistry were performed for morphological observations of the brain tissues in all animals, and then the activity of NF-KBp65 was detected using enzyme-linked immunosorbent assay. Results The expressions of NF-KBp65 and COX-2 were observed in brain issues with EAE. Significant differences were found in the number of cells positive for NF-KBp65 and COX-2 between EAE groups and the control group. NF-KBp65 and COX-2 expressions were associated with the symptoms of EAE. In addition, significantly increased activity ofNF-kBp65 was noted in EAE, which is immuned with R-SCH. Conclusion NF-KB and COX-2 are expressed in rabbits with EAE and play important roles in the pathogenesis of EAE.
