Impact of cell transplantation on glutamate and dopamine in the rat striatum
10.3760/cma.j.issn.1671-8925.2008.11.011
- VernacularTitle:细胞移植术对大鼠纹状体内谷氨酸和多巴胺的影响
- Author:
Ling LIN
1
;
Yu-Hong ZHENG
;
Zhi-Hong ZHENG
Author Information
1. 福建医科大学
- Keywords:
Cell transplantation;
Brain micredialysis;
Neurotransmitter;
HPLC
- From:
Chinese Journal of Neuromedicine
2008;7(11):1127-1130
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the impact of anesthetization and manipulation on neurotransmitters glutamate and dopamine during cell transplantation. Methods Neural stem cells cultured in vitro from postnatal rats were implanted into the striatum of normal adult rats. Brain microdialysis combined with high-performance liquid chromatography (HPLC) was applied to dynamically detect the impact of microdialysis probe implantation, anesthetics ketamine and pentobarbital and implanted cells etc on glutamate and dopamine levels. Results After 15 min probe implantation into the rat striatum, glutamate and dopamine levels in the striatum increased, evidently higher than the baseline value and declined to the baseline level within about 5-6 h. Ketamine and pentobarbital anesthetization for 15 rain resulted in a transient increase in glutamate and dopamine levels in the rat striamm;there were no significant effects on in vivo glutamate and dopamine levels by cell implantation itself. Conclusion Routine doses of intraperitoneal ketamine or pentobarbital anesthetization may result in a transient increase in glutamate and dopamine levels within the brain extracellular space. Based on these data, the optimal time for commencing brain microdialysis on glutamate and dopamine should be at least 6 h after probe implantation.
