Correlation of interleukin-8 expression to astrocytic tumor cell proliferation and apoptosis

VernacularTitle:白细胞介素8表达与星形细胞肿瘤增殖与凋亡的相关性研究
Author: Bao-Dong CHEN ¹ ; Ru-Xiang XU ; Xiao-Dan JIA ; Yong-Zhong GAO ; Tai-peng NG JIA ; Jian-Jun DING ; Wei-Ping LI
Author Information

1. 南方医科大学珠江医院
Keywords: lnterleukin-8; Proliferating cell nuclear antigen; Apoptosis; Astrocytic rumor
From: Chinese Journal of Neuromedicine 2008;7(11):1120-1123
CountryChina
Language:Chinese
Abstract: Objective To investigate the expressions of interleukin-8 (IL-8) mRNA and proliferating cell nuclear antigen (PCNA) protein in astrocytic tumors and their correlation with the tumor cell apoptosis. Methods The expressions of IL-8 mRNA in 64 cases of astrocytic tumor and 10 normal brain tissues were detected using RT-PCR. The expression of PCNA protein in the tumors was assayed with immunohistochemistry, and the apoptosis index (AI) of the tumor cells determined using TUNEL assay. Results The expression ofIL-8 mRNA in the astrocytic tumors was strongly correlated to the malignancy of the tumors, and both the PCNA expression and the apoptosis index increased in tumors of greater malignancies. IL-8 mRNA expression was positively correlated to the expression of PCNA (r=0.938, P<0.01) and AI (r=0.907, P<0.01) in these tumors. Conclusion IL-8 mRNA expression is positively correlated to the histological grade of the astrocytic tumors, whose proliferation is mediated by inhibition of cell apoptosis and upregulation of IL-8 transcription.