Curcumin inhibits the expression of pituitary tumor transforming gene in glioma C6 cell line in vitro
10.3760/cma.j.issn.1671-8925.2008.11.002
- VernacularTitle:姜黄素对胶质瘤C6细胞垂体瘤转化基因下调作用的体外研究
- Author:
Liang-Qun RONG
1
;
Ru-Fei DAI
;
Jun CAI
;
Zai-Hai YAN
;
Chao YAN
Author Information
1. 徐州医学院第二附属医院
- Keywords:
Glioma;
Curcumin;
Pituitary Tumor Transforming Gene
- From:
Chinese Journal of Neuromedicine
2008;7(11):1087-1089
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of cnrcumin on the expression of pituitary tumor transforming gene (PTTG) in glioma C6 cell line. Methods Glioma C6 cells were allocated into the control group and 3 cttrcnmin treatment groups with curcumin treatment at 10, 20 and 30 μmol/L for 24 h. After the treatments, PTTG mRNA and protein expressions in the cells were detected by reverse transcription-polymerase chain reaction (RT-PCR) and Western blotting, respectively. Results The expression levels of PTTG mRNA in the 4 groups, showed significant differences between any two groups (P<0.01). Significant differences were also found in PTTG protein expressions between the 4 groups of C6 cells after the treatment (P<0.01). Conclusions Cureumin can down-regulate PTTG expression at both the mRNA and protein levels in glioma C6 cells in a dose-dependent manner.
