Anti-inflammatory and immune-modulating effects of Dihuang Heji in the treatment of multiple sclerosis
10.3760/cma.j.issn.1671-8925.2008.09.015
- VernacularTitle:地黄合剂在多发性硬化患者中抗炎性反应及调节免疫平衡机制探讨
- Author:
Cong GAO
1
;
Fu-Hua XIE
;
Teng-Fei OU
;
Jie YANG
;
Yan-Song SHENG
;
Ming GAO
Author Information
1. 广州医学院附属第二医院
- Keywords:
Dihuang Heji;
Glial fibrillary acidic protein;
S100B;
Multiple sclerosis;
CD4+ cells;
CD8+ cells
- From:
Chinese Journal of Neuromedicine
2008;7(9):923-927
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the anti-inflammatory and immune-modulating effects of Dilmang Heji(DHHJ),a compound traditional Chinese medicinal preparation,in patients with multiple sclerosis(MS)and explore the possible mechanisms underlying these effects. Methods Forty MS patients were randomized into prednisone treatment and prednisone+DHHJ group for the corresponding treatments.Another 20 surgical patients without immune or inflammatory diseases undergoing lumbar anesthesia served as the control group.Glial fibrillary acidic protein(GFAP)and S100B levels in the cerebrospinal fluid(CSF)and the peripheral blood of these subjects were detected using ennzynle-linked immtmosorbent assay(ELISA), and the numbers 0f CD4+ and CD8+ cells were detected by flow cytometry.The ambulation index(AD,expanded disability status scale(EDSS)and 9-hole PEG test (9HPT)were used to assess the patients'clinical symptoms.All the patients were followed up for3 years to record the number of times of MS relapse. Results GFAP and S100B levels in the CSF were significantly higher in the MS patients than in the healthy subjects,but lower in MS patients treatedwith prednisone plus DHHJ thanin those with prednisone treatment only(P<0.05).In the MS patients,AI and 9HPT scores were correlated to the GFAP and S100B levels in the CSF.Irednisone plus DHHJ treatment was associated with significantly reduced MS relapse in comparison with prednisone treatment alone(P<0.05).Before the treatment,the MS patients showed increased CD4+ cell number and decreased CD8+ cell number especially in the CSF;atter the treaRnents,the CD4+ ceils decreased and CD8+ cells increased,and this effect was stronger with prednisone plus DHHJ treatment(P<0.05).Conclusions DHHJ produces anti-inflammatory effect by inhibiting glial cell activation and modulating immune balance in MS,thus alleviating the symptoms of MS and reducing MS relapse.DHHJ may provide anideal adjuvanttherapy for MS.
