Effect of organophosphate-induced acute neuronal damage in rats
10.3760/cma.j.issn.1671-8925.2008.09.013
- VernacularTitle:急性有机磷酸酯类化合物中毒对大鼠神经元的损伤作用
- Author:
Xin-Ying FAN
1
;
Wu-Sheng ZHU
;
Long YANG
;
Shu RONG
;
Ge-Lin XU
;
Ren-Liang ZHANG
;
Min-Min MA
;
Yun LI
;
Xin-Feng LIU
Author Information
1. 南京军区南京总医院
- Keywords:
Organophosphorus compounds;
Brain injuries;
Neuronal nuclear antigen
- From:
Chinese Journal of Neuromedicine
2008;7(9):915-918
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate the neuronal injury induced by organophosphorus(OP) compound exposurein rats andinvestigate andthepossible mechanisms. Methods Eighteen SD rats were randomly divided into OP groups(n=12)and the control group(n=6).SD rats were given intramuscular sarin inection followed 1 min later by intraperitoneal injection of atropine sulphate and pralidoxime,and the rats with typical toxic reactions were used for subsequent experiment.The rats in the control group received normal saline injections in identical manners.Twenty-four hours later,the brain tissue of the rats were taken for HE staining and neuronal nuclei antigen(NeuN)immunohistochemistry to quantitatively assess the neuronal damages in the pyriform cortex,hippocampus CAl and striatum.Results HE staining showed massive degeneration of the neurons in the pyriform cortex,hippocampus CAland striatum of rats with satin injection.Compared to the rats with saline injections,the rats exposed to satin presented with significantly decreased number of NeuN-positive neurons(P<0.05).Conclusion OP Can induce acute neuronal death in rat brain and cause a series of symptoms in the central nervous system,probably by such noncholinergic mechanisms as glutamic acid-induced eytotoxieity and oxidative stress.
