Tumor necrosis factor-related apoptosis-inducing ligand induces glioma U87 cell apoptosis through the mitochondrial apoptotic pathway
10.3760/cma.j.issn.1671-8925.2008.09.004
- VernacularTitle:肿瘤坏死因子相关凋亡诱导配体诱导胶质瘤细胞凋亡机制研究
- Author:
Fei ZHONG
1
;
Chun-Kui SHAO
;
Jin-Xiang LIN
;
Jing YANG
;
Xiang-Yuan WU
;
Qu LIN
;
Ming DONG
;
Jing-Yun WEN
;
Xiao-Kun MA
;
Li WEI
Author Information
1. 中山大学附属第三医院
- Keywords:
Tumor necrosis factor-related apoptosis-inducing ligand;
Glioma;
Apoptosis;
Mitochondria
- From:
Chinese Journal of Neuromedicine
2008;7(9):878-880,885
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the mechanism of tumor necrosis factor-related apoptosis-inducing ligand(TRAIL)-induced apoptosis of glioma U87 cells.Methods Human glioma U87 cells were treated with human recombinant soluble TRAIL(rsTRAIL),and the cell apoptosis was detecmd with flow cytometry with AnnexinV-FITC/PI double staining.Flow cytometry with DiOC6 staining was used to assess the changes in mitochondrial transmembrane potential(△ψm).The relative activity of caspase-3,-8 and-9 Was measmed by colorimetric assay,and the concentration of cytoplasmic cytochrome C(cyt C) determined using enzyme-linked immunosorbem assay.The effects ofcaspase-8 inhibitor(Z-IETD-fmk)on rsTRAIL-induced apoptosis,△ψm,caspase-3,-8 and-9 activities and cyt C concentration were observed. Results RsTRAIL tinle-dependently induced apoptosis and progressive collapse of △ψm in glioma U87 cells,resulting also in caspase-3,-8 and-9activation and elevated cytC concentration.Caspase-8 inhibitor partially antagonized these biological effects induced by rsTRAIL in U87 cells.Conclusion TRAIL initiates a cascade of mitochondrial events by activating caspase-8 and induces apoptosis of glioma U87 cells.
