Effects of salvia miltiorrhizae injection on hypoxia-induced apoptosis or cultured rat neuronal stem cells and activity of Caspase-3
10.3760/cma.j.issn.1671-8925.2008.08.009
- VernacularTitle:丹参注射液对缺氧神经干细胞凋亡和Caspase-3活性的影响
- Author:
Tao HUANG
1
;
Fu HAN
;
Zhi-Qiang ZHANG
;
Qi-Jia TAN
;
Cai-Jun XIE
;
Shao-Ying XIE
;
Can-Hui ZHU
Author Information
1. 广东省中医院
- Keywords:
Salvia miltiorrhizae;
Neuronal stem cells;
Hypoxia;
Apoptosis;
Caspase-3
- From:
Chinese Journal of Neuromedicine
2008;7(8):793-795
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the effects of salvia miltiorrhizae (SM) injection on the apoptosis of cultured rat neuronal stem cells induced by hypoxia and the activity of Caspase-3, in order to provide the further evidence for the molecular mechanism of neuroprotection of SM injection. Methods The neuronal stem cells from neonatal rat hippocampus were cultured and divided randomly into normal control group, hypoxia group and SM treatment group. After Hoechst staining, the apoptotic morphological change and apoptosis percentage were observed under fluorescence microscope. The activities of Caspase-3 in the 3 groups were evaluated by the colorimetric assay. Results Compared with normal control group [(2.75±0.28)%, 1.16±0.07], the percentage of apoptosis and the activity of Caspase-3 were increased significantly in neuronal stem cells cultured in hypoxia [(30.12%±2.09)%,3.85±0.41, P<0.05). Application of SM injection reduced markedly the percentage of apoptosis and the activity of Caspase-3 of the neuronal stem cells cultured in hypoxia [(9.16±1.34)%, 1.50±0.09, P<0.05].Conclusion SM injection can depress the apoptosis of the rat neuronal stem cells induced by hypoxia,so as to exert the neuroprotection.
