Relationship between G protein signal transduction pathway and intracranial aneurysm
10.3760/cma.j.issn.1671-8925.2008.07.014
- VernacularTitle:G蛋白信号传导通路和颅内动脉瘤的关系
- Author:
Gang ZHU
1
;
Zhong-Song SHI
;
Yong-Hua ZHU
;
Ming-Chang LI
;
Yi-Ming SUN
;
Hua-Hao DAI
;
Wei-Sheng PAN
;
Zheng-Song HUANG
Author Information
1. 中山大学附属第一医院
- Keywords:
Intracranial aneurysm;
Signal transduction pathway,G protein;
Gene expressionprofiling
- From:
Chinese Journal of Neuromedicine
2008;7(7):698-701
- CountryChina
- Language:Chinese
-
Abstract:
Objective To identify G protein signal transduction pathway related genes in peripheral blood mononuclear cells and aneurysm tissues from intracranial aneurysm patients using oligo-microarrays. Methods Blood samples from 50 intracranial aneurysms and 10 healthy human donors were obtained after informed consent. Peripheral blood mononuclear cells (PBMCs) were isolated from blood using Ficoll method. And aneurysm tissue samples from 6 intracraniai aneurysms and 3 healthy human intracranial arteries were obtained. The cDNA were retrotranscribed from the extracted RNA and the biotin-labeled cRNA derived from the transcription of cDNA were fragmented as probes. Then, the probes were hybridized with Affymetrix Human Genome UI33A Array. GeneArmy Scanner was used to screen the signals of hybridization and Microarray Suite software 5.0 was applied to analyze the expression of genes. Results There were 325 genes differently expressed in PBMCs from intracranial aneurysm patients compared to healthy human. And 571 genes were differently expressed above 2-fold level in aneurysmal tissue from intracranial aneurysm patients compared to healthy human intracranial arteries. Function analysis was confirmed from upregulated genes and downregulated genes, and among these genes, there were several genes invovled in G protein signal transduction pathway. There were 3 genes directionally expressed (RA831 upregulated and ARHGAP8, CENTG2 downregulated) both in PBMCs and aneurysm tissues from intracranial aneurysm patients. Conclusions The methodologies of genechip will provide a new powerful approach to help identify the molecular mechanisms of intracranial aneurysm. G protein signal transduction pathway may be the possible mechanism for the pathogenesis of intracranial aneurysms in Chinese.
