Changes of CtyC and AIF in brain and effects of them on neuronal cells in rats after traumatic brain injury
10.3760/cma.j.issn.1671-8925.2008.07.012
- VernacularTitle:创伤性脑损伤后大鼠脑组织CtyC与AIF的变化及其对神经细胞的影响
- Author:
Wen-Jun QU
1
;
Ru-Xiang XU
;
Ying-Qian CAI
;
Dong-Jian HUANG
Author Information
1. 广东省第二人民医院
- Keywords:
Craniocerebral trauma;
Cytochromes C;
Apoptosis-inducing factor;
Apoptosis
- From:
Chinese Journal of Neuromedicine
2008;7(7):691-694
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the changes of cytochrome C (CtyC) and apoptosis-induced factor (AIF) in the brain and effects of them on neuronal cells in rats after traumatic brain injury (TBI). Methods Seventy Sprague-Dawley (SD) rats were randomly divided into sham-injury group (n=10) and brain injury group. The brain injury group was divided into 6 subgroups according to 1, 6, 24, 48, 72, 168 h after traumatic brain injury (n=10). Rat models with contusion and laceration of brain were established by Feeney's impact with flee falling. Fluorescence intensity of CtyC and AIF were observed by immnnofluorescence and confocal laser scanning microscopy at different time points. The effects of CtyC and AIF on neuronal cells were observed by double-labeled immunofluorescence. Results (1)The changes of CtyC in the brain after TBI: at 1, 6, 24 and 48 h following TBL CtyC was 1.89±0.03, 2.69±0.07, 2.99±0.06 and 3.05±0.05 in the cortex and 1.34±0.04, 1.87±0.03, 2.60±0.03 and 2.80±0.06 in the hippocampus, respectively, being significantly higher than that of sham-injury group (P<0.05); at 72 h, CtyC declined to 1.94±0.05 in the cortex and to 1.12±0.04 in the hippocampus, respectively, being significantly higher than that of sham-injury group (P<0.05); at 168 h, CtyC recovered to the normal level in the cortex and hippocampus, being not significantly different between injury group and sham-injury group. (2) The changes of AIF in the brain after TBI: at 1, 6, 24 and 48 h following TBI, AIF was 1.82± 0.16, 2.16±0.34, 2.75±0.22, 2.87±0.12 in the cortex and 1.27±0.06, 2.01±0.05, 2.49±0.02, 2.62±0.05 in the hippocampus, respectively, being significantly higher than that of sham-injury group (P<0.05); at 72h, AIF declined to 1.35±0.09 in the cortex and to 1.32±0.05 in the hippocampus, respectively, being significantly higher than that of sham-in jury group (P<0.05); at 168 h, AIF recovered to the normal level in the cortex and hippocampus, being not significantly different between injury group and sham-injury group (P<0.05). (3) CtyC and AIF triggered neuronal cell apoptosis. Conclusion CtyC and AIF might release from mitochondria after TBI, and can induce neuronal cell apoptosis as apoptosis-inducing factors.
