Preventive effects of ischendc preconditioning and penehyclidine hydrochloride on spinal cord against ischemia-reperfusion injury in rabbits
10.3760/cma.j.issn.1671-8925.2008.07.010
- VernacularTitle:缺血预处理和长托宁防治脊髓缺血再灌注损伤的实验研究
- Author:
Qi-Bin KE
1
;
Ya JIANG
;
De-Hai SUN
;
Qing-Hun LI
;
Guang-Feng GE
Author Information
1. 三峡大学第一临床医学院(湖北省宜昌市中心人民医院)
- Keywords:
Ischemic preconditioning;
Penehyclidine hydrochloride;
Spinal cord;
Ischemia-repeffusion injury
- From:
Chinese Journal of Neuromedicine
2008;7(7):684-687,697
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the protective effect of ischemic preconditioning (IPC) and penehyclidine hydrochloride (PHC) on the spinal cord against ischemia-reperfusion injury induced by aortic cross-clamping in rabbits. Methods Twenty-four rabbits were randomly divided into sham operation group (A), ischemia-reperfusion injury group (B) and IPC+PHC group (C). In group C, IPC was performed for 5 min, followed by reperfusion for 30 min, and PHC (0.2 mg/kg) was given at 20 min of rcperfusion. Then, in group B and C, the infrarenal aorta was clamped for 40 min followed by 7 d reperfusion. The aorta was not clamped in group A. The plasma concentrations of malondialdehede (MDA), superoxide dismutase (SOD), creatine kinase (CK) and creatine kinase BB isoenzyme (CK-BB) were assayed at 10 min before clamping, before unclamping, at 60 min and on the 7 th day after unclamping. After an operation, the neurological outcomes of the hind limbs were evaluated, the morphology of the spinal cord was observed, and the apoptotic spinal cord cells were measured by immunohistochemical technique. Results The concentrations of MDA, CK and CK-BB after ischemia-reperfusion in group B were increased significantly compared with those before clamping and those in group A (P<0.05 or 0.01); the concentrations in group C were higher than those before clamping (P<0.05), but lower significantly than those in group B (P<0.01), and not significantly different from those in group A. SOD was opposite. The apoptotic cells in group B were much more than that in groupA and in group C, but the number in group C was significantly higher than that in group A (P<0.01). The incidence rate of paralysis in group C was significantly lower than that in group B, and the neurological score of hinder limb was higher in group C than in group B (P<0.01). Pathological changes of the spinal cord was milder in group C than in group B. Conclusion The combination of IPC and PHC can protect the spinal cord from ischemia-reperfusion injury in rabbits; the main mechanism may be increasing antioxidant potential and preventing cell injury.
