Expressions of MMP-9 and TIMP-1 after focal cerebral ischemia-reperfusion in rats
10.3760/cma.j.issn.1671-8925.2008.07.004
- VernacularTitle:大鼠局灶性脑缺血再灌注后MMP-9、TIMP-1的表达
- Author:
He-Cheng CHEN
1
;
Jin-Sheng YANG
;
Xiao-Han DING
;
Xiang-Qun SHI
;
Yin-Sheng YANG
Author Information
1. 兰州军区兰州总医院
- Keywords:
Cerebral iscbemia-reperfusion;
MMP-9;
TIMP-1
- From:
Chinese Journal of Neuromedicine
2008;7(7):661-664
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the expressions of matrix metalloproteinase-9 (MMP-9) and tissue inhibitors of matrix metalloproteinase-1 (TIMP-1) after focal cerebral ischemia-repeffusion injury in rats. Methods Totally 40 Wistar rats were randomly divided into 2 groups: sham-operated group and iscbemia-reperfusion group. A middle cerebral artery occlusion model was constructed in the 25 rats with Longa's method. The expressions of MMP-9 and TIMP-1 were investigated with immunohistochemistry and HE staining at 6, 24, 48, 72 h and 7 d of reperfusion following 2 h ischemia. Results MMP-9 began to be expressed at 6 h reperfusion, and was obviously increased at 24 h and reached the peak level at 48 h, and then, the expression of MMP-9 began to decrease at 72 h to a low level at 7 d. TIMP-1 positive cells began to arise at 6 h reperfusion, peaked at 24 h, decreased at 48 h and remained a low level at 7 d. The expressions of MMP-9 and TIMP-1 were mainly located in vascular endothelial cells, neurons and gitter cells. The expressions of MMP-9 and TIMP-1 were negative in sham-operated group. Conclusion The expressions of MMP-9 and TIMP-1 are induced to increase by focal cerebral ischemia-reperfusion, and vascular endothelial injury may be the main cause to the high expressions of MMP-9 and TLMP-1.
