Detection of Ca2+i in neuron-like cells from bone mesenchymal stem cell differentiation induced by salvia miltiorrhiza
10.3760/cma.j.issn.1671-8925.2008.06.016
- VernacularTitle:丹参诱导大鼠骨髓间充质干细胞分化为神经元样细胞中的钙离子检测
- Author:
Tao HUANG
1
;
Fu HAN
;
Zhi-Qiang ZHANG
;
Cai-Jun XIE
;
Shao-Ying XIE
;
Qi-Jia TAN
;
Xiao-Chuan LUO
;
You-Bi SHEN
Author Information
1. 广东省中医院
- Keywords:
Bone mesenchymal stem cells;
Neuron;
Intracellular flee Ca2+;
Salvia miltiorrhiza injection;
Induction and differentiation
- From:
Chinese Journal of Neuromedicine
2008;7(6):603-606
- CountryChina
- Language:Chinese
-
Abstract:
Objective To measure the concentration of intracellular free Ca2+ ([Ca2+]i) in neuron-like cells resulted from rat bone mesenchymal stem cell (BMSCs) differentiation induced by salvia miltiorrhiza injection and provide some theoretical basis for the BMSCs transplantation. Methods The rat BMSCs were separated from rat bone marrow and cultured in vitro. After induced by basic fibroblast growth factor and 10mL/L salvia miltiorrhiza injection, the cells were identified with immunofluorescence staining against NeuN. The same procedure was performed on primarily cultured hippocampal neurons. Then, the [Ca2+]i of the differentiated neuron-like cells was determined and compared with primarily cultured hippocampal neurons. Results The BMSCs after induced by basic fibroblast growth factor and salvia miltiorrhiza injection expressed neuronal phenotypes similar to the cell appearance of neurons with NeuN. The average fluorescence intensity of the neuron-like cells derived from BMSCs was 984.75±79.51, while the average fluorescence intensity of the primarily cultured hippocampal neurons was 769.42±60.93. No significant difference was found between them (P>0.05). Conclusion The neuron-like cells from rat BMSCs differentiation induced by salvia miltiorrhiza injection possess certain neuronal properties.
